![]() septempunctata feeding on the artificial diet accumulated amino acid metabolic pathways, lipid metabolic pathways, and starch and glucose metabolism were down-regulated. Furthermore, in the most DEGs metabolic pathways for C. septempunctata observed in the group fed the artificial diet. These differentially expressed genes (DEGs) were likely associated with the decreased egg laying capacity, hatching rate, longevity, and increased sex ratio (♀:♂) of adult C. Comparison of transcriptome sequences revealed that expression of 38,315 genes was affected by the artificial diet, and 1,182 of these genes showed a significant change in expression levels (FDR ≤ 0.05,|log2FC|≥1, "FC" stands for "fold change"). septempunctata libraries, and the clean reads were assembled using Trinity de novo software (Tabel 2). The Illumina HiSeq2000 was used to sequence 691,942,058 total clean reads from artificial diet-fed and A. septempunctata that were differentially expressed depending on diet. In this study, we used transcriptome sequencing analysis to identify nutrition- and metabolism-related genes of C. fecundity, egg hatching rate, survival rate, etc.) compared with those fed natural prey (Aphis craccivora), likely due to different nutritional characteristics of the diet. septempunctata fed an artificial diet showed diminished biological properties(e.g. The insect predator Coccinella septempunctata can effectively control many types of pests, such as aphids, whiteflies, and small lepidopteran larvae.
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